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bghp polya  (Addgene inc)


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    Structured Review

    Addgene inc bghp polya
    Bghp Polya, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bghp+polya/pAAV-synP-FLEX-splitTVA-EGFP-B19G+(Plasmid+%2352473)/pmc09243523__mmc6-199-24-27
    Average 93 stars, based on 24 article reviews
    bghp polya - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Construct:

    Article Title: A versatile viral toolkit for functional discovery in the nervous system
    Article Snippet: .. VTKS/D constructs containing the promoter and inducible sites were synthesized (see ) and cloned using ApaI/AscI into a backbone containing ITRs, WPRE and bGHp polyA (from Addgene #52473, a generous gift from Ian Wickersham) ( ). .. For VTKS1-hChR2-tBFP, we cloned the hChR2 coding sequence from the Addgene plasmid #26976 (a generous gift from Karl Deisseroth) and tBFP from the Addgene plasmid #80086 (a generous gift from Connie Cepko); for VTKS2-hM3Dq-P2A-tBFP, we amplified the hM3Dq-P2A coding sequence from Addgene plasmid #83897 ( ) and tBFP from the Addgene plasmid #80086; for VTKS3-TeTLc-P2A-mCherry, the coding sequence of TeTLc-P2A-mCherry was a generous gift from Rosa Cossart; for VTKD4-V5-APEX2-NES and VTKD6-V5-APEX2-NES, we amplified the V5-APEX2-NES coding sequence from the plasmid Addgene #72480 (a generous gift from Alice Ting); for VTKS5-N2cG-P2A-eGFP-P2A-TVA, we amplified the eGFP-P2A-TVA coding sequence from Addgene plasmid #52473 (a generous gift from Tom Jessell) and we cloned N2cG from pAM-CAGGS-FLEX-H2B-GFP-P2A-N2c(G ) ( ).

    Article Title: A versatile viral toolkit for functional discovery in the nervous system.
    Article Snippet: .. VTKS/D constructs containing the promoter and inducible sites were synthesized (see Table S2) and cloned using ApaI/AscI into a backbone containing ITRs, WPRE and bGHp polyA (from Addgene #52473, a generous gift from Ian Wickersham) (Kohara et al., 2014). .. Transgenic mice, SST-Cre (stock number: 013044), SST-FlpO (stock number: 031629), Lhx6-iCre (stock number: 026555), ACTB-FlpE (stock number: 005703), ACTB-Cre (stock number: 003376), ChAT-Cre (stock number: 031661), Sun1-GFP (stock number: 021039), RCE (stock number: 32037), Cas9eGFP (stock number: 026179) are available at Jackson Laboratories.

    Synthesized:

    Article Title: A versatile viral toolkit for functional discovery in the nervous system
    Article Snippet: .. VTKS/D constructs containing the promoter and inducible sites were synthesized (see ) and cloned using ApaI/AscI into a backbone containing ITRs, WPRE and bGHp polyA (from Addgene #52473, a generous gift from Ian Wickersham) ( ). .. For VTKS1-hChR2-tBFP, we cloned the hChR2 coding sequence from the Addgene plasmid #26976 (a generous gift from Karl Deisseroth) and tBFP from the Addgene plasmid #80086 (a generous gift from Connie Cepko); for VTKS2-hM3Dq-P2A-tBFP, we amplified the hM3Dq-P2A coding sequence from Addgene plasmid #83897 ( ) and tBFP from the Addgene plasmid #80086; for VTKS3-TeTLc-P2A-mCherry, the coding sequence of TeTLc-P2A-mCherry was a generous gift from Rosa Cossart; for VTKD4-V5-APEX2-NES and VTKD6-V5-APEX2-NES, we amplified the V5-APEX2-NES coding sequence from the plasmid Addgene #72480 (a generous gift from Alice Ting); for VTKS5-N2cG-P2A-eGFP-P2A-TVA, we amplified the eGFP-P2A-TVA coding sequence from Addgene plasmid #52473 (a generous gift from Tom Jessell) and we cloned N2cG from pAM-CAGGS-FLEX-H2B-GFP-P2A-N2c(G ) ( ).

    Article Title: A versatile viral toolkit for functional discovery in the nervous system.
    Article Snippet: .. VTKS/D constructs containing the promoter and inducible sites were synthesized (see Table S2) and cloned using ApaI/AscI into a backbone containing ITRs, WPRE and bGHp polyA (from Addgene #52473, a generous gift from Ian Wickersham) (Kohara et al., 2014). .. Transgenic mice, SST-Cre (stock number: 013044), SST-FlpO (stock number: 031629), Lhx6-iCre (stock number: 026555), ACTB-FlpE (stock number: 005703), ACTB-Cre (stock number: 003376), ChAT-Cre (stock number: 031661), Sun1-GFP (stock number: 021039), RCE (stock number: 32037), Cas9eGFP (stock number: 026179) are available at Jackson Laboratories.

    Clone Assay:

    Article Title: A versatile viral toolkit for functional discovery in the nervous system
    Article Snippet: .. VTKS/D constructs containing the promoter and inducible sites were synthesized (see ) and cloned using ApaI/AscI into a backbone containing ITRs, WPRE and bGHp polyA (from Addgene #52473, a generous gift from Ian Wickersham) ( ). .. For VTKS1-hChR2-tBFP, we cloned the hChR2 coding sequence from the Addgene plasmid #26976 (a generous gift from Karl Deisseroth) and tBFP from the Addgene plasmid #80086 (a generous gift from Connie Cepko); for VTKS2-hM3Dq-P2A-tBFP, we amplified the hM3Dq-P2A coding sequence from Addgene plasmid #83897 ( ) and tBFP from the Addgene plasmid #80086; for VTKS3-TeTLc-P2A-mCherry, the coding sequence of TeTLc-P2A-mCherry was a generous gift from Rosa Cossart; for VTKD4-V5-APEX2-NES and VTKD6-V5-APEX2-NES, we amplified the V5-APEX2-NES coding sequence from the plasmid Addgene #72480 (a generous gift from Alice Ting); for VTKS5-N2cG-P2A-eGFP-P2A-TVA, we amplified the eGFP-P2A-TVA coding sequence from Addgene plasmid #52473 (a generous gift from Tom Jessell) and we cloned N2cG from pAM-CAGGS-FLEX-H2B-GFP-P2A-N2c(G ) ( ).

    Article Title: A versatile viral toolkit for functional discovery in the nervous system.
    Article Snippet: .. VTKS/D constructs containing the promoter and inducible sites were synthesized (see Table S2) and cloned using ApaI/AscI into a backbone containing ITRs, WPRE and bGHp polyA (from Addgene #52473, a generous gift from Ian Wickersham) (Kohara et al., 2014). .. Transgenic mice, SST-Cre (stock number: 013044), SST-FlpO (stock number: 031629), Lhx6-iCre (stock number: 026555), ACTB-FlpE (stock number: 005703), ACTB-Cre (stock number: 003376), ChAT-Cre (stock number: 031661), Sun1-GFP (stock number: 021039), RCE (stock number: 32037), Cas9eGFP (stock number: 026179) are available at Jackson Laboratories.

    other:

    Article Title: A versatile viral toolkit for functional discovery in the nervous system
    Article Snippet: Transgenic mice, SST-Cre (stock number: 013044), SST-FlpO (stock number: 031629), Lhx6-iCre (stock number: 026555), ACTB-FlpE (stock number: 005703), ACTB-Cre (stock number: 003376), ChAT-Cre (stock number: 031661), Sun1-GFP (stock number: 021039), RCE (stock number: 32037), Cas9eGFP (stock number: 026179) are available at Jackson Laboratories.



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